New strategy of two-dimensional gel electrophoresis-based proteomics analysis in esophageal cancer
- VernacularTitle:以双向凝胶电泳为基础的食管癌组织蛋白质组学分析新策略
- Author:
Lishun WANG
;
Yanhui YIN
;
Sujun GAO
;
Al ET
- Publication Type:Journal Article
- Keywords:
Proteomics;
Esophageal cancer;
2DE
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To apply the strategy of proteomics with two dimensional gel electrophoresis(2DE) in esophageal cancer.Methods:the protein of esophageal cancer tissues and its adjacent normal tissue were separated by 2DE, the gels were analyzed with software ImageMaster4.01 to find out the spots different between cancer and control, and the proteins in the spots were identified by matrix associated laser desorption/ionization time of flight(MALDI TOF) mass spectrometry.Results:a new reasonable strategy of study for proteomics on esophageal cancer was raised and candidate cancer marker of two pyruvate kinase isozyme were found under such strategy.Conclusion:Under reasonable strategy, the analysis of proteomics with 2DE on human tissue is a useful method for discovering valuable cancer marker candidates. Pyruvate kinase isozyme M2/M1 which were found in cancer tussues will be very important in diagnosis and treatment of esophageal cancer.
