Construction of a eukaryotic expressing vector containing CP15 gene of C.parvum and its expression in Hela cells
- VernacularTitle:微小隐孢子虫CP15基因高效真核表达载体的构建及其在Hela细胞中的表达
- Author:
Hongxuan HE
;
Xichen ZHANG
;
Hongsheng OUYANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Crypstosporidium parvum;
CP15;
Eukaryotic expression vector;
Hela cells
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct an eukaryotic expressing vector pCR3 1 15 containing CP15 gene of Crypstosporidium parvum(C.parvum) and express it in Hela cells Methods:CP15 gene of C parvum was obtained from pMD18 T 15 disgested by BglⅡ and was inserted into eukaryotic expressing vector pCR3 1(+) in BamHⅠ site,and then Hela cells were transfected with recombinant by liposomes The transcription and expressed products of CP15 in the transfected Hela cells were assayed by RT PCR,ELISA and indiret immunofluorescence assay after screening with G418 Results:It showed that pCR3 1 15 was constructed successfully CP15 gene was transcripted in transfectants and CP15 protein with obvious biological activity was highly expressed in Hela cells Conclusion:CP15 gene in recombinant vector is proved to be expressed in Hela cells and obvious biological activity of expression production in transfected cells was detected
