Enhancing effect of IL-18 gene modification on the induction of antitumor immunity by antigen pulsed dendritic cells
- VernacularTitle:IL-18基因修饰增强肿瘤抗原多肽致敏的树突状细胞体内诱导的抗肿瘤免疫反应
- Author:
Jiquan CHEN
;
Xuetao CAO
;
Qingyu XIU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Tumor antigen peptide;
IL 18;
Dendritic cells;
CTL;
Immunotherapy;
Gene therapy
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to study the antitumor immune response induced by antigen pulsed,IL 18 gene modified dendritic cells in vivo.Methods:①C57BL/6 mice were immunized twice subcutaneously by tumor antigen peptide pulsed,IL 18 gene modified dendritic cells(DC IL 18/mut1)(1?10 5 cells/mouse),then the NK activity and CTL activity were determined.②In block test,C57BL/6 mice were first immunized once 2?10 5 cells/mouse DC IL 18/mut1 subcutaneously,and then challenged by 5?10 5 3LL Lewis lung cancer cells/mouse with blocking different immune components by monoclonal antibody,the tumor growth were observed.Results:Specific CTL activity and NK activity could be induced most significantly in mice immunized with DC IL 18/mut1.The block test showed that CD4 +T,costimulating pathway,the production of IFN ? involved in the induction phase of immunization with DC IL 18/mut1,and CD8 +T?IFN ??NK cells involved in the effect phase but CD4 +T cells unnecessary.Conclusion:Immunization with DC msIL 18/mut1 could induce potent antitumor immune activity,whose mechanisms involved effective antigen presentation,increased CTL activity and NK activity,CD4 +?CD8 +T?NK cells incorporation,and the production of IFN ?.
