The immune response of CD8~+T cells from subjects of different HLA-A~*02 subtype detected by intracellular cytokine staining
- VernacularTitle:用细胞内细胞因子染色方法检测HLA-A~*02个体CD8~+T细胞的免疫应答
- Author:
Ruobing LI
;
Hongsong CHEN
;
Dan LI
;
Al ET
- Publication Type:Journal Article
- Keywords:
Cytokine;
HLA A 02 subtype;
Immune response
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Intracellular cytokine staining(ICCS) is applied to the detection of the immune response of peripheral blood mononuclear cells (PBMC)from subjects of different HLA A 02 supertype to influenza A matrix peptide.Methods:HLA A 02 subtype were identified by the way of SSP methods;PBMC from subjects with HLA A 02 were cocultured with influenza A matrix peptide (IMP) which was HLA A*0201 restricted CTL epitope, secreting cytokine such as IL 2, IFN ?,TNF ? were measured by ICCS.Results:PBMC from HLA A 02 positive subjects had memory immune response to IMP.Conclusion:①The method of ICCS can be used for quantitive detecting T cell activating status specificly;②the memory immune response to IMP58 66 peptide exists in all of the HLA A 02 positive persons;③among different HLA A 02 subtype, there are cross reactivity to HLA A 0201restricted peptide.
