Analysis for misassignments of HLA-B homozygotes by serology and detection for HLA-B expression variants
- VernacularTitle:血清学指定HLA-B座位纯合型误差分析及HLA-B座位表达变异检测的意义
- Author:
Jiong BI
;
Qian FU
;
Xiaowei LI
;
Al ET
- Publication Type:Journal Article
- Keywords:
HLA B homozygotes;
Polymerase chain reaction;
Serological typing;
Expression variants
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find the discrepancies caused by HLA B gene variants in HLA B antigen homozygotes which were typed again by DNA method.Methods:75 blood samples assigned HLA B homozygotes by serology were typed and detected HLA B expression variants by PCR SSP.Results:The 12 samples had been assigned the second alleles by DNA method in 75 HLA B homozygotes by serology.One out of 12 samples was identified expression variant by PCR SSP.The null allele was caused by the insertion of an extra cytosine at the beginning of exon 4.Conclusion:HLA B gene typing by PCR SSP will be the use of supplement to serology.The expression variant detection by PCR SSP will improve accuracy for tissue typing and benefit clinical application.
