Role of plasmid DNA and in combination with IFN-? or with CH50 in activation of macrophages immunity
- VernacularTitle:质粒DNA及其协同IFN-?、CH50在巨噬细胞免疫功能激活中的作用
- Author:
Hui XIAO
;
Zuohua FENG
;
Guimei ZHANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Plasmid;
Macrophage;
Synergistic effect;
Immune activation
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of eucaryotic expression plasmid DNA on activation of macrophage and the mechanism Methods:NO production by macrophages,the expression of surface molecules on macrophage by FACS and the cytotoxicity of macrophage were measured by Griess method,FACS and MTT respectively Results:The results showed that macrophages were activated to produce NO by plasmid pCH510 and pcDNA3 1 respectively in vitro or in vivo The expression of MHC Ⅱand B7 1 on macrophage and the cytotoxicity of macrophage was increased Plasmids DNA in combination with IFN ? but not with CH50 augmented the activation of macrophage in vitro In contrast, the activation of the macrophage were enhanced when the stimulated macrophages by plasmid DNA in vivo were treated by addition of CH50, but not IFN ? Conclusion:The results suggested the plasmid DNA could activate macrophage in vitro or in vivo but the mechanism of activation was different In vitro, the plasmid DNA directly activated macrophage and had synergistic effect with IFN ?,while in vivo macrophages were mainly indirectly activated and the activation of macrophages was enhanced in the presence of CH50 When eucaryotic expression plasmid pCH510 was used in gene therapy of tumor, besides the function of its products expressed, the plasmids showed the significant role in tumortherapy by its direct immune activation of macrophages
