A preliminary study on targeting antigenic epitopes of monoclonal antibody against human TNF-?
- VernacularTitle:抗人TNF-?单克隆抗体抗原识别表位的初步研究
- Author:
Wei ZHANG
;
Beifen SHEN
;
- Publication Type:Journal Article
- Keywords:
hTNF ?;
Monoclonal antibody;
Antigenic epitope;
Deletion mutant
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To locate the region of antigenic epitopes recognized by monoclonal antibody(Z8) against human tumor necrosis factor ?(hTNF ?).Methods:Several recombinant fusion expression vectors were constructed which contained hTNF ? mutant genes deleted different parts respectively. After induction with IPTG, the fusion proteins were expressed and analyzed by SDS PAGE and Western blot.Results:Z8 specially recognized the fusion products which contained amino acids from 92 to 157 of hTNF ?, but not GST and its fusion protein with amino acids from 1 to 91 of hTNF ?.Conclusion:The antigenic epitopes recognized by Z8 were located in the sequence region 92 157 of hTNF ?.
