The inhibition effect of human chorionic gonadotropin(hCG) on mRNA expression of cytokines initiated inflammatory reaction
- VernacularTitle:人绒毛膜促性腺激素(hCG)抑制人PBMC促炎细胞因子mRNA的表达
- Author:
Hong BAI
;
Jufen PAN
;
Xiaoqing JIA
;
Al ET
- Publication Type:Journal Article
- Keywords:
Human chorionic gonadotropin(hCG);
TNF?;
IL 1;
IL 6;
Gene expression
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of hCG on the gene expression of cytokines(TNF??IL 1 and IL 6) that initiate inflammatory reaction protected against bacteria.Methods:Human PBMC were cultured with LPS(22 ?g/ml) and different doses of hCG(100,50,25,12 5,6 25,3 125 U/ml) at 37℃,5%CO 2 for 4 h,mRNA of cells harvested were isolated,then run RT PCR with primer of TNF??IL 1 and IL 6 separately.The PCR product were quantitated by using MIAS 2000 analyzer,calculated the OD value.Results:Compared with the control group,the result showed that hCG in a dose range of 50～6.25 U/ml have significantly suppressive effect on TNF? mRNA expression ( P
