The relationship between point mutation of nt3394 T→C of mitochondrial DNA and type 2 diabetes mellitus in the elderly
- VernacularTitle:线粒体DNA ND1基因nt3394 T→C突变与老年人2型糖尿病
- Author:
Pei YU
;
Demin YU
;
Demin LIU
;
- Publication Type:Journal Article
- Keywords:
DNA,mitochondrial;
DNA mutational analysis;
Diabetes mellitus, non insulin dependent
- From:
Chinese Journal of Geriatrics
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between point mutation of nt3394 T→C of mitochondrial DNA ND1 and type 2 diabetes mellitus(DM) in the elderly. Methods Two hundred and eight patients with type 2 diabetes mellitus and 180 subjects with normal glucose tolerance without DM family history were included. The mutation was determined by polymerase chain reaction and restriction fragment length polymorphism(PCR RFLP). Results The point mutation of nt3394 T→C of mitochondrial DNA ND1 was found in 9 of 208 elderly patients with type 2 DM(4 3%), and 1 of 180 controls (0 6%). The incidence of this mutation was significantly different between the two groups ( P
