Isolation of differently expressed cDNAs from the hippcampus of rats with vascular dementia by RNA fingerprinting
- VernacularTitle:RNA指纹法分离血管性痴呆大鼠海马内的差异表达基因
- Author:
Xuezhao ZHANG
;
Guojie SUN
;
- Publication Type:Journal Article
- Keywords:
Dementia,vascular;
Hippocampus;
Gene expression
- From:
Chinese Journal of Geriatrics
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate differently expressed cDNAs associated with vascular dementia from hippocampus of rats. Methods The creation of vascular dementia model of rat was by 4 vessel occlusion method. The learning and memory of vascular dementia(VaD) rats were examined by Morris water maze. RNA fingerprinting was used to analyse the differently expressed cDNAs in the hippocampus of normal aging and VaD rats. At the same time, cDNAs expressed differently during the progression of VaD were isolated. Results Thirty two candidate cDNA fragments were isolated by RNA fingerprinting. The two cDNA fragments specifically expressed in VaD rats were analysed by Northern blot. Homology analysis through BLAST revealed that these two were novel genes and were given numbers of BG937392 and BG937393 in the gene bank. Conclusions RNA fingerprinting is a simple and quick method for isolating differently expressed genes. These two new gene fragments may contribute significantly to the process of VaD as pathogenic genes or protective genes.
