The effect of endogenous transforming growth factor ?_1 on the growth of bladder cancer cells in vitro
- VernacularTitle:内源性转化生长因子?_1对膀胱癌细胞体外生长的影响
- Author:
Xin YAO
;
Wenlu LI
;
Jiwu CHANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Transforming growth factor ? 1 Cell line;
Proliferation
- From:
Chinese Journal of Urology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of endogenous transforming growth factor ? 1 (TGF? 1) on the cell cycle regulation and proliferation of bladder cancer. Methods A constructed replication defective retroviral vector pRevT? AS,which carried antisense RNA of TGF? 1,was transfected to a bladder cancer cell line EJ.The proliferation and clone formation of transferred cells were observed in vitro,and the alteration of cell cycle was also detected by flow cytometric analysis. Results TGF? 1 antisense RNA was transferred into EJ cell and expressed efficiently.After the inhibition of target gene expression in EJ cells,the reduced growth and clone formation rates were demonstrated,and the proliferative indexes were decreased by 12%.The ratios of G 0 and G 1 stage cells to the antisense RNA transfected EJ cells were increased,simultaneously,the ratio of S stage cells to the antisense RNA transfected EJ cells ratios were decreased,compared with the control group. Conclusions The proliferative mechanism of endogenous TGF? 1 in bladder cancer cells is to stimulate the G 1 to S stage transition.
