Immune-related gene expression profile in multiple sclerosis with gene chips
- VernacularTitle:多发性硬化免疫相关基因表达谱的基因芯片研究
- Author:
Guiqin SONG
;
Weiping WU
;
Ke ZHU
;
- Publication Type:Journal Article
- Keywords:
Multiple sclerosis;
Immunity,cellular;
Antibody formation;
Gene expression;
Oligonucleotide array sequence analysis
- From:
Chinese Journal of Neurology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the immune genes expression profile database of multiple sclerosis (MS) and to screen the high immune related candidate genes by using microarray analysis Methods Polymerase chain reaction (PCR) products on behalf of 484 immune related genes from mixed tissue library were assembled on the modified Oako glass slide The general RNA from MS patients and normal persons lymphocytes was transcripted to cDNA by RT PCR and labelled with cy 3 and cy 5 The two probes were mixed and hybridized with the above mentioned gene chips Scan array 4000 was used for scanning the hybridizing signals and GenePix Pro3 0 for date analysis Results Of the total 484 double genes monitored,22 genes of group one showed changes in expression level of the ratio outside 0 5 and 2.0;27 genes differ in group 2;72 genes change in group 3 With the same consistent gene ID of the double genes, the group one was 6,the group tow was 9 and the group three was 30 Conclusions These results show that the different expression of immune related genes might occur between MS patients and normal persons It might also afford a view of the changes that these genes should be probably related to the occurrence, development or progress of MS The cell immune related genes might be the important and most immune related genes involved in MS pathogenesis The results suggest the deeper insights into the mechanism,relapsing and treatment of MS
