CpG ODN enhance the antibody production in mice immunized with hepatitis B surface antigen
- VernacularTitle:CpG ODN增强乙型肝炎表面抗原免疫小鼠的抗体产生
- Author:
Yanchun SHI
;
Guizhen YANG
;
- Publication Type:Journal Article
- Keywords:
CpG ODN;
HBsAg;
Vaccine;
Adjuvant
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of synthetic oligodeoxynucleotide containing CpG motifs (CpG ODN) on antibody production to recombinant hepatitis B surface antigen (rHBsAg) or hepatitis B vaccine respectively. Methods:The antigens were injected into the back leg tibialis anterior(TA) muscle of all the mice (Km breed and Balb/c breed) for two times.The serum anti HBs titers were detected by ELISA.Results:The anti HBs titers of additional CpG ODN groups are obviously higher than those of rHBsAg group or vaccine group alone, the antibody persistent time is some longer. Nevertheless, the anti HBs titers of pure breeding mice are significantly higher than those of non pure breeding mice. Conclusion:CpG ODN has enhanced efficacy for anti HBs production in immunized mice and synergetic effect can be observed in alum adjuvant of hepatitis B vaccine.
