Effects of c-myc antisense oligodeoxyribonucleotides(ASODNS) on the expression of surface antigen of the tumor cells and susceptibility of target cells to CD_3AK attack
- VernacularTitle:c-myc反义寡核苷酸对肿瘤细胞表面抗原和对CD_3AK杀伤敏感性的调节作用
- Author:
Zhaozhong LI
;
Ling ZHANG
;
Yun WANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
c myc;
Antisense oligodeoxyribonucleotides(ASODNS);
HLA ABC;
ICAM 1;
CD 3AK
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the role and mechanism of c myc ASODNS on cell expression of surface antigen of the tumor cells and susceptibility of target cells to immune cells attack.Methods:c myc mRNA was examined by RT PCR.MTT assay was usd to explore the effects of c myc ASODNS on PG cell sentisentity to lysis by CD 3AK effector cells.The expression of HLA ABC,ICAM 1,c myc protein was examined by flow cytometry.Results:When PG cells were treated with ASODNS(1 ?mol/L) there was a markably reduction of expression of c myc protein.Expression ratio of HLA ABC and ICAM 1 surface antigen expression ratio of PG cells were enhanced from 68.44%,38.44% to 83.16% and 42.09% respectively( P
