BsAb HER-2?CD3 mediated the cytotoxicity of T lymphocytes to the breast cancer cells overexpressing HER-2
- VernacularTitle:BsAb HER-2?CD3对过度表达HER-2乳腺癌细胞的细胞毒作用
- Author:
Guanjun WANG
;
Limin WEN
;
Lijun MA
;
Al ET
- Publication Type:Journal Article
- Keywords:
BsAb;
HER 2;
CD3;
Cytotoxicity
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To induce the specific cytotoxicity of T lymphocytes to the breast cancer cells overexpressing HER 2 by bispecific monoclonal antibodies anti HER 2?anti CD3 and to stimulate natural immunoreactions. Methods:Using SK BR 3 cell line overexpressing HER 2 as target cells, MCF 7 cell line as control cells, and T lymphocytes as effector cells, the biological function of BsAb anti HER 2?anti CD3 was studied, including growth inhibition of target cells, proliferation of effect cells, and cytotoxicity of lymphocytes mediated to SK BR 3. The measuring methods included MTT(measurement of tetrazplium), 3H thymidine incorporation assay system.Results:BsAb anti HER 2?anti CD3 had the function of MAbHER 2 and MAbCD3, which inhibited growth of SK BR 3 and accelerated proliferation of T lymphocytes from normal donors. BsAb anti HER 2?anti CD3 mediated more significant cytotoxicity of T lymphocytes to SK BR 3 than MAb HER 2.The best ratio of effector cells to target cells was 20∶1. Conclusion:BsAb anti HER 2?anti CD3 bound to the breast cancer cells overexpressing HER 2 and T lymphocytes and mediated significant cytotoxicity of T lymphocytes to the breast cancer cells overexpressing HER 2, indicating BsAb anti HER 2?anti CD3 had significant antitumor function.
