Detection for DNA Damage in Human Lymphocytes Induced by Four Chemicals Using Comet Assay
- VernacularTitle:彗星试验对4种化学物质诱导人淋巴细胞DNA损伤的检测
- Author:
Dong-Ping YANG
;
Mei-Bian ZHANG
;
Ji-Liang HE
;
Al ET
- Publication Type:Journal Article
- Keywords:
Comet assay;
DNA damage;
4NQO;
MMS;
Bleomycin;
Mitomycin
- From:
Journal of Environment and Health
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To validate feasibility of comet assay as a tool for detecting DNA damage induced by various types of chemical mutagens.Study of DNA damage induced by4chemicals on human lymphocytes was carried out in vitro.Methods Human lymphocytes were exposed to4-nitroquinoline-1-oxide(4NQO,a UV-mimetic agent ),methyl methanesulfonate(MMS,an alkylating agent ),Bleomycin(BLM,a radiamimetic agent )and Mitomycin(MMC,a DNA crosslink agent )for3h,the DNA single strand breaks(SSB)induced by4chemicals were measured immediately(0h-incubation)and21h-incubation after3h-exposure to the chemicals with comet assay.Results It was found that the SSB induced by4NQO,MMS and BLM,which revealed a dose-response relationship(P
