High level expression of 6His-hEra
- VernacularTitle:6His-hEra融合蛋白的高效表达
- Author:
Zongling JI
;
Sumin CHEN
;
Jizhong LIU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Human Era GTP binding protein Expression Sequence
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of human Era.Methods:The encoding region of human Era gene was inserted into pUC19 plasmid and sequenced,the gene fragment was then cloned into the prokaryotic fusion expression vector pRSET B to contruct recombinant expression plasmid pRSET B hEra.The recombinant protein was expressed in E.coli BL21DE3(plys S).Results:6His hEra was expressed after induced by IPTG and took 51.2% of the total cell lysate and mainly existed in the inclusion body.Conclusion:High level of human Era was established and this result may shed light on the further study on function of human Era.
