HLA-DR genotyping and clinical application using Microarray DNA Chip
- VernacularTitle:HLA-DR位点的基因芯片分型与临床应用
- Author:
Chengtao LI
;
Ying YANG
;
Li LI
;
Al ET
- Publication Type:Journal Article
- Keywords:
HLA DR Genotyping DNA Chip PCR SSP PCR SSO
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a new method performed on an DNA chip for genotyping HLA DR and supply a new view.Methods:According to the particular sequence of HLA DR exon2,HLA DR genotyping Chip was made,then the labeled PCR products hybridized with them,the signals were sanned by sanner and analyzed by Imagene software.70 standard DNA and 200 donor recipients have been genotyped and some of samples have been sequenced.Results:The experimental results showed that the HLA DR genotyping chip made are accurate and sensitive.Thirty alleles of HLA DR were accurately distinguished and its overall time of DNA typing was 3 hours.Conclusion:This proved that the DNA Microarray technique is good for DR genotyping and high resolution,high specificity,well reproducibility.Compared with PCR SSP and PCR SSO methods,the genotyping chip method is more intuitionistic and has the advantage of integration.It can also genotype HLA A,B alleles and many persons in a chip at the same time.It is more suitable for clinical application and establishment of marrow bank and umbilical cord bank.
