Effect of isoflavone and genistein on expression of CD69 by activated T lymphocytes in vitro
- VernacularTitle:异黄酮Genistein对T细胞体外活化CD69表达的影响
- Author:
Jingxian ZHAO
;
Jieming ZENG
;
Yaoying ZENG
;
- Publication Type:Journal Article
- Keywords:
Genistein T lymphocyte Activation
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of isoflavone and genistein on activation of T lymphocytes in order to develope new immuno intervention reagent.Methods:Fluorescence conjugated monoclonal antibodies and flow cytometer were used to detect the expression rate of CD69 by activated T cells in vitro in response to Phytohemagglutinin(PHA) and Phorbol 12,13 dibutyrate(PDB),with some samples pre incubated with 10,50 or 100 ?mol/L of genistein,after 2 h and 6 h of incubation in whole blood culture system.Results:After 2 h of culture,the inhibitory effect in PHA group was stronger than PDB group(P
