Preparation and determination of polyclonal antibody to pre-mRNA splicing protein Tra2?
- VernacularTitle:前体mRNA剪接蛋白Tra2?特异抗体的制备和鉴定
- Author:
Xianhua CHEN
;
Wanmin LIN
;
Kaihua SUN
;
Al ET
- Publication Type:Journal Article
- Keywords:
Tra2? Pre mRNA splicing protein Specific antigen fragment Antibody
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare specific antibody against the pre mRNA splicing protein Tra2? in human fetal tissues.Methods:A specific antigen fragment of human Tra2? was cloned into the pGEX 3x expression vector to express a recombinant GST Tra2? fusion protein.The fusion protein was expressed,purified and used as an antigen to immunize the New Zealand rabbit,and the polyclonal antibody to Tra2? was obtained.Results:The antibody was successfully used to detect Tra2? protein in human fetal cerebrum with Western blot and immunohistochemistry analysis.Conclusion:This prepard antibody has good specificity and can be used to detect the Tra2? protein in the human fetal tissues.
