An experimental study of vascular endothelial growth factor and transforming gro wth factor a_1 expression in bone defect resulted from non-union
- VernacularTitle:VEGF、TGF-?_1在骨缺损不愈合中表达的实验研究
- Author:
Xinlong MA
;
Jun XIE
;
Pei WANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Fractures,ununited;
Endothelial growth factors;
Trousformi ng growth factors beta;
Gene expression_
- From:
Chinese Journal of Orthopaedics
1996;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of vascular endothelia l growth factor (VEGF)and transforming growth factor a 1 (TGF-a 1 )in callus of non-union,bone defect for investigation of the molecular mechanisms of bone defect resulted from non-union,and further explore the co rrelative influences of VEGF and TGF-a 1 in fracture healing.Methods The bone defect resulted from non-union model and the fracture healing model were established in the radius of rabbit.The sp ecimens of callus and soft tissue were collected postoperatively at1,2,4, 6,8and12weeks.The expressions of VEGF and TGF-a 1 were in-vestigated by means of immunohistochemistry(SP method).Results There was no sign of subperiosteal healing except a large segment filled with hemorrhage and granulation tissue in bone defect area.There was only a little amount of granulation tissues transforming into chondrocytes.Some chondrocyt es stopped prolif-erating after reaching hyper-trophic chondrocyte stage but changed into fibrous connective tissues.Most of the granulation tissue finally changed to fibrous connective tissues in the fracture gap.In addition,there w ere a large number blood vessels invading the normal fracture healing site than that in non-union.In im muno-histochemistry observation,there were significa nt decreasing expressions of VEGF and TGF-a 1 in callus of different period of non-union comparing to union(P
