Regulatory study of LBP on the expression of T cells apoptosis-related genes in aged rats
- VernacularTitle:枸杞多糖调控老年大鼠T细胞凋亡及相关基因表达的研究
- Author:
Ziyin SHEN
;
Weimin GUO
;
Yu CHEN
;
- Publication Type:Journal Article
- Keywords:
Immunosenescence Apoptosis Gene expression Lycium Barbarum Polysaccharide
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory mechanism of LBP on T lymphocytes excessive apoptosis and the mRNA expression of apoptotic genes in aged rats Methods:T lymphocyte apoptosis and the expression of pre apoptotic and anti apoptotic genes (Fas, FasL, TNFR1, Bax, Bcl2 and TNFR2) from young rats and old rats treated with or without LBP were studied using flow cytometry and fluorescence real time quantitative RT PCR Results:LBP could effectively downregulate the percentage of T cell apoptosis in aged rats At the same time LBP also downregulated the transcription level of TNFR1 gene and upregulated the transcription level of Bcl 2 in T cells from old rats Conclusion:The T cell excessive apoptosis in old rats could be effectively downregulated with LBP, by the by of decreasing the expression of pro apoptotic gene and enhancing the expression of anti apoptotic gene
