Expression of telomerase activity in peripheral blood of patients with lung cancer
- VernacularTitle:肺癌患者血中循环癌细胞端粒酶活性的表达及意义
- Author:
Guangli FENG
;
Jiuxian FENG
;
Qianggang DONG
;
Al ET
- Publication Type:Journal Article
- Keywords:
lung neoplasma;
telomerase;
tumor markers;
circulating cancer cell
- From:
China Oncology
1998;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To detect the telomerase activity of lung cancer cells in peripheral blood by TRAP.Methods:The mononuclear cell fraction in peripheral blood was isolated by Ficoll Hypaque gradient centrifugation. Then the telomerase activity of cancer cells in peripheral blood of 49 pre chemotherapy patients was determined by PCR TRAP. Results:In the 49 cases, 65.3%(32/49) cases showed significantly increased telomerase activity, among these cases the telomerase activity was especially high in small cell lung cancer and advanced non small cell lung cancer patients, and telomerase activity in the primary chemotherapy patients was higher than in the recurrent chemotherapy patients. Conclusions:Telomerase activity may be a tumor marker of cancer cells in peripheral blood and can evaluate chemotherapy response in lung cancer.
