Hypoxia-induced apoptosis and anti-apoptosis in ovarian cancer cell line SKOV-3ipl cells and its correlation with cell cycle
- VernacularTitle:卵巢癌细胞株SKOV-3ipl缺氧与凋亡及其与细胞周期的关系
- Author:
Yaping ZHU
;
Youjie FENG
;
Yafang CHEN
;
Al ET
- Publication Type:Journal Article
- Keywords:
ovary;
malignancy;
cell lin;
hypoxia;
apoptosis;
cell cycle
- From:China Oncology
1998;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate hypoxia induced apoptosis and anti apoptosis in ovarian cancer cell line SKOV 3ipl cells and its correlation with cell cycle changes.Methods:The monolayer SKOV 3ipl cells plated in culture bottles were placed in an Anaerobic system insulated with 85% N 2,5% CO 2,10% H 2 and very low O 2 concentration (100 ppm). Apoptosis and cell cycles were measured with FITC Annexin V/PI double stain and DNA prep stain Flow cytometry in cells cultured in anaerobic condition. Results:Apoptosis in cells cultured in hypoxia condition for 0h,8h,16h,24h. were (0.32?0.01)%,(8.29?0.87)%,(4.23?0.75)%,(0.59?0.08)% respectively. Between 8h or 16h and 0h, P
