Isolation of genes that are induced by Ty21a in intestinal cells by suppression subtractive hybridization (SSH)
- VernacularTitle:利用抑制消减杂交技术(SSH)研究与Ty21a免疫相关的基因
- Author:
Hua WANG
;
Jie-Ying GAO
;
Jie ZHOU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Ty21a Suppression subtractive hybridization cDNA microarray Intestinal cells
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate the genes induced by Ty21a. Methods: Prepare the intestinal cells of Balb/C mouse with and without Ty21a immunization, extract the polyA+ RNA, synthesize the dscDNA by using reverse transcription-PCR,get the differentially expressed genes by suppression subtractive hybridization(SSH).Results:Seven genes were novel genes revealed by GenBank searching whose expression were probably induced by Ty21a were isolated by SSH and cDNA microarray. The full length cDNA sequence of three genes are now obtaining through RACE-PCR. Conclusion: SSH is an effective method for isolating the differentiatlly expressed genes; High throughput cDNA microanay is a credibility method to profile changes in gene expression; Ty21a can induce the expression of some new genes related to immune.
