Combinational effects of bcl-2 antisense oligodexynucleotide with arsenic trioxide on malignant lymphoma cell lines
- VernacularTitle:bcl-2基因的反义寡核苷酸与三氧化二砷联合对恶性淋巴瘤细胞系凋亡的影响
- Author:
Lin NIE
;
Yuan ZHANG
;
Jianjun ZHANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
arsenic trioxide;
lymphoma;
bcl 2 gene;
antisense oligodeoxynucleotide
- From:
China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate the effects of bcl 2 antisense phosphorothioate oligodeoxynucleotides (ASODN) combined with arsenic trioxide on the malignant lymphoma cell lines.Methods:Apoptosis, cellular DNA contents and bcl 2 expression were determined by cell stain, TdT mediated dUTP Nick end Labeling (TUNEL) and flow cytometry. Results:10～40?mol/L bcl 2 ASODN and 0.5～2.0 ?mol/L As 2 O 3 could inhibit Raji cell growth and induce apoptosis significantly. Number of Sub G 1 cells significantly increased and Bcl 2 protein expression in Raji cells significantly was downregulated by flow cytometry assay. The relationship between level of bcl 2 protein and concentrations of As 2 O 3 and bcl 2 ASODN showed a dose and time dependent effect; so did the number of Sub G 1 cells. There is much more effective inhibition of growth and induction of apoptosis by their combination than alone. Conclusions:It is found that bcl 2 ASODN could significantly inhibit the growth of B lymphoma cell lines and induce their apoptosis when combined with arsenic trioxide.
