A monoclonal antibody 1F9 against Torpedo Acetylcholinesterase recognizes specifically an antigenic epitope of the human brain Acetylcholinesterase
- VernacularTitle:特异识别人脑乙酰胆碱酯酶抗原表位的单克隆抗体1F9
- Author:
Qian LI
;
Wei LIU
;
Junhua WU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Epitope Human brain Acetylcholinesterase ELISA McAb Decapeptide library
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to research the relationship between the function and the structure of human brain Acetylcholinesterase,use the monocolonal antibodies scanning antigenic decapeptides of human brain Acetylcholinesterase.Methods:Synthesis of overlapping decapeptides corresponding to the sequence of the human brain Acetylcholinesterase has been carried out on biotinylated with primary amino groups according to a method developed by M Geyson. Peptides Synthesized using the multipincombinatorial chemical synthesis technique have been used in an enzyme linked immunoabsorbent assay.Results:A antigenic epitope of human brain Acetylcholinesterase recognized by monoclonal antibody against Torpedo Acetylcholinesterase is received by decapeptide scanning.Conclusion:The antigenic epitope 111 could be recognized by the polycolonal antibody against human brain Acetylcholinesterase and monoclonal antibody against Torpedo Acetylcholinesterase,the results indicate that the epitope is highly conserved in human brain AChE and Torpedo AChE.
