Characterization of antigenicity of the outer membrane protein from Edwardsiella tarda
- VernacularTitle:迟缓爱德华菌外膜蛋白抗原性分析
- Author:
Xinxin HUANG
;
Chengping LU
;
- Publication Type:Journal Article
- Keywords:
Edwardsiella tarda Outer membrane protein Antigen 2
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the antigenicity of OMP extracted from Edwardsiella tarda.Methods:ELISA, Bactericidal test, Agglutinating test and Western blotting were used for testing the antigenic titers and immunogenicity of OMP.Results:In immunoblotting, by using ATCC15947 OMP antibody, the non pathgenic strains were negative, while all pathogenic strains except Et 122 gave positive results and had OMP bands of 33k, 35k, 38k, and 45k. OMPs of both ATCC 15947 and JEL4 could induce high antibody titers. Further more, the antibodies evoked by OMPs of ATCC 15947 of 33k or 35k could also protected mice to some degree when diluted.Conclusion:The 33k, 35k, 38k, and 45k of OMPs may be protective antigens, and the OMPs of Et could be a candidative component for vaccine.
