Effect of antineoplastic polypeptide from Buthus martensii venom on human tumor cell lines and animal transplanting tumors
- VernacularTitle:东亚钳蝎毒抗癌多肽对人肿瘤细胞系和动物移植瘤的作用
- Author:
Ling WEI
;
Weihua DONG
;
Tianhan KONG
;
- Publication Type:Journal Article
- Keywords:
scorpion venom;
leukemia;
liver neoplasm;
transplating tumor;
antineoplastic agents;
cultured tumor cell line
- From:China Oncology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate the effect of antineoplastic polypeptide from Buthus martensii venom (APBMV) on the cultured human promyelocytic leukemia cells HL 60 and hepatoma cell line SMMC 7721 and hepatoma H 22 bearing mice.Methods:MTT colorimetric method, growth inhibiting test and colony formation assay were used in the in vitro test. H 22 bearing mice were applied in the in vivo experiment. Through measuring tumor growth inhibitory rate(IR),white blood cell (WBC) number and spleen index (SI) ,we explored the influence of APBMV on H 22 bearing mice.Results:APBMV possessed stronger cytotoxicity on HL 60 cells and SMMC 7721 cells, and IC 50 was 10.74 ?g/ml and 11.33 ?g/ml , respectively. APBMV could dramatically inhibit their growths. There were obvious dosage response correlations. The IC 50 of HL 60 and SMMC 7721 at 24h, 48h and 96h were 19.41 ?g/ml, 9.90 ?g/ml, 11.41 ?g/ml and 15.87 ?g/ml, 13.05 ?g/ml, 8.70 ?g/ml, respectively. When the concentration of APBMV exceeded 8 ?g/ml, the colony formation rate of SMMC 7721 cells decreased dramatically ( P 0.05).Tumor growth of H 22 bearing mice was markedly inhibited by APBMV,the growth inhibiting rate was reached 40.30% ( P
