Expression of TopoⅡ gene in human bladder cancer multidrug-resistant cells
- VernacularTitle:人膀胱癌耐药细胞中TopoⅡ基因的表达
- Author:
Yuanning ZHANG
;
Tangjun YANG
;
Xiangwei WANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
bladder cancer;
DNA topoisomerase Ⅱ;
multidrug resistance;
reverse transcriptase polymerase chain reaction
- From:China Oncology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study the expression of TopoⅡ gene in multidrug resistant cells of human bladder cancer. Methods:The degree of drug resistance was detected by MTT method ;the expression of TopoⅡ gene in cell lines BIU 87/ADM and BIU 87 was detected with reverse transcriptase polymerase chain reaction. Results:The cell lines BIU 87/ADM were 56.4 times more resistant to ADM than the cell lines BIU 87;the expression of TopoⅡ gene was poorly positive in BIU 87/ADM but strongly positive in BIU 87 cells. Conclusions: The decreased expression of TopoⅡ gene in BIU 87/ADM cells might contribute to the development of multidrug resistance of human bladder cancer.
