Xenograft of microencapsulated human pancreatic islets to diabetic mice
- VernacularTitle:微囊化人胎胰岛移植治疗小鼠实验性糖尿病的观察
- Author:
Hong LAI
;
Li CHEN
;
Chuanlong WU
;
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Islands of langerhans;
Transplantation;
Mice
- From:
Chinese Journal of Current Advances in General Surgery
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the efficacy of microcapsules to prolong islet xenografts survival in mice.Methods:Human fetal pancreatic islets were isolated from the embryo which was obtained from legal abortion(gestational age 16～24 weeks) with collagenase and enclosed in semipermeable alginate BaCl 2 capsules.Diabetic BALB/ C mice induced with streptozotocin were divided into 3 groups.Each group had 7 mice.Then transplantation was performed.Results:Transplantation of 1000?100 encapsulated fetal islets into the peritoneal cavities of 7 BALB/ C mice restord normalglycemia for 78.4?21.27 days without immunosuppression.The second group of 7 diabetic mice received an equal number of uncultured pancreatic fragments.These unprotected xenografts were functional for only 7.43?3.42 days,but high mortality occured.There was significant differences between the two groups(P
