Preparation of SEREX-defined tumor antigens and preliminary study of their sero-reactivity
- VernacularTitle:SEREX肿瘤抗原基因表达蛋白的研制和血清学初步筛选
- Author:
Huizhen ZHANG
;
Ying WANG
;
Ming YUAN
;
Al ET
- Publication Type:Journal Article
- Keywords:
OVA SEREX detection Tumor antigen Serology
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain tumor antigens for study of the sero activity in sera of normal subjects and tumor patients.Methods:Had cloned MY OVA 2,7 and 13 full length genes and expressed the fusion proteins in E.Coli.The proteins were characterized by SDS PAGE and Western Blot.Using bacterially synthesized and purified proteins,74 patients with different kinds of tumors and 13 healthy controls by dot blot were investigated.Results:Expressed fusion proteins at high level,reaching a yield at least 30% of the total bacteria protein.By using affinity chromatography and thrombin digestion,finally gained three purified proteins,which could be applied to serum screening.Dot blot results showed that auto antibodies against MY OVA 2 and MY OVA 7 were detectable in some of tumor and normal samples,while antibody against MY OVA 13 was only detected in tumor patients(5/74) and not detected in normal subjects(0/13).Conclusion:Tumor antigens prepared from genetic engineering can be adopted for effective seroscreening.
