The modulatory effect of Chinese herb substances BP on apoptosis of lymphocytes in the elderly
- VernacularTitle:中药成分BP对老年人淋巴细胞凋亡的调控作用
- Author:
Junda LIU
;
Shu WANG
;
Li CAO
;
Al ET
- Publication Type:Journal Article
- Keywords:
Chinese herb BP Lymphocytes Apoptosis CD45RO positive cells Automatic image analysis
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the changes of lymphocytes subpopulation and apoptosis process of lymphocytes in the elderly and to investigate the modulatory effect of BP from Chinese herb on apoptosis.Methods:Lymphocytes phenotype were determinated using indirect immunofluorescence technique.The proliferative responses were measured with MTT method.Apoptosis of lymphocytes was evaluated by flow cytometry and automatic image analysis.Results:The proliferative responses of lymphocytes in the elderly were lower than that of young adults.Decreased CD45RA positive cells and increased CD 45 RO positive cells were found in lymphocytes population of aged people compared to that of young adults. The CD 45 PO positive cells were prone to apoptosis.There is an imhibitory effect of BP on apoptosis of lymphocytes in the elderly.Conclusion:It was implicated that the susceptibility of lymphocyte in the elderly to apoptosis depends on activation,so called activation induced cell death.Present results suggested that apoptosis of lymphocytes of aged people play an important role in the pathogenesis of immunosenescence.The possibility appeared for development of modulatory drugs on apoptosis from Chinese herbs.
