APOPTOSIS INDUCED BY TNF-? COMBINED WITH 5-Fu AND WITH EXPRESSION OF BCL-2 GENE IN COLON CANCER CELLS
- VernacularTitle:TNF和5-Fu联合诱导结肠癌细胞凋亡及bcl-2基因表达的实验研究
- Author:
Zhihong ZHOU
;
Jianwei ZHENG
;
Shaohong SHANG
;
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor ?;
5 Fu;
Apoptosis;
Colorectal cancer;
bcl 2 gene
- From:
Cancer Research and Clinic
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of recombined TNF ? combined with induction of apoptosis and its relation with expression of bcl 2 in SW480 human colon cancer cells.Methods:The inhibitive effect of 5 Fu and TNF ? was measured by MTT.Apoptosis of colon cancer cells was studied by TUNEL.The expression of bcl 2 gene was determined by immunohistochemical analysis.Results:After SW480 colon cancer cells were treated with combination in various concentration of 5 Fu and 3 000 U/mL of TNF ? for 48 h,MTT assay revealed a significant decrease of relative viability in compasrison to the control.When SW480 colon cancer cells were treated with 3 000 U/mL of TNF ? and 125 ug/mL of 5 Fu in combination for 48 h,TUNEL method showed the number of nick end positive cells was significantly increased in comparison to the control.The expression level of bcl 2 gene lowered significantly than that of the control group.Conclusion:These results confirmed that combined TNF ? and 5 Fu could synergistically induce apoptosis in SW480 cells and decrease the expression level of bcl 2 gene.
