The assay of apoptosis of PBMC and lymphocyte subsets in chronic hepatitis B
- VernacularTitle:慢性乙型肝炎PBMC凋亡及淋巴细胞亚群的检测
- Author:
Ling LI
;
Changhai GU
;
Xin LI
;
Al ET
- Publication Type:Journal Article
- Keywords:
Chronic hepatitis B Chronic severe hepatitis B AICD Lymphocyte subset
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to explore the AICD happening in PBMC and the situation of peripheral blood lymphocyte subsets in chronic/ chronic severe hepatitis B Methods:The peripheral blood mononuclear cells of patients with chronic /chronic severe hepatitis B were cultured with PHA P for 72 h Then the apoptosis of PBMC was assayed by flow cytometry The peripheral blood lymphocyte subsets of patients with chronic /chronic severe hepatitis B were assayed by flow cytometry and automatic blood analyzer Results:The percentage of apoptotic PBMC in chronic hepatitis B group was higher than that in chronic severe hepatitis B group(P
