Changes in the Expression of Bone Morphogenetic Protein Receptor Type IA, IB and II in Human Prostate by the Suppression of Dihydrotestosterone.
- Author:
Soo Mee KWON
1
;
Sun Il KIM
;
Jong Ran LEE
;
Dong Hyun LEE
;
Sung Joon HONG
Author Information
1. Department of Urology, College of Medicine, Yonsei University, Seoul, Korea. sjhong346@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Benign prostatic hyperplasia;
Bone morphogenetic protein;
Receptor;
Dihydrotestosterone;
Prostate
- MeSH:
Bone Morphogenetic Protein Receptors;
Bone Morphogenetic Proteins*;
Dihydrotestosterone*;
Finasteride;
Humans*;
Immunoblotting;
Middle Aged;
Polymerase Chain Reaction;
Prostate*;
Prostatic Hyperplasia;
RNA, Messenger;
Transurethral Resection of Prostate
- From:Korean Journal of Urology
2002;43(12):1045-1050
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate whether the expression of type IA, IB and II bone morphogenetic protein receptors (hBMPRs) are affected by the suppression of dihydrotestosterone (DHT) in the prostate tissues of patients with benign prostatic hyperplasia (BPH), we determined mRNA levels and protein expression patterns of the hBMPRs in human prostate tissues. MATERIALS AND METHODS: Frozen tissues were obtained during the transurethral resection of the prostate (TURP) in BPH patients who had taken the 5alpha-reductase inhibitor (finasteride), for 3 months prior to surgery, for the reduction of the prostate volume. Tissues from patients who had not taken finasteride prior to the TURP were used as controls. Patients over 50 years old, and with a prostate volume over 50ml, were included. Semiquantitative polymerase chain reaction (PCR) and immunoblotting were used to compare the expressions of the human bone morphogenetic protein receptors (hBMPRs) between the experimental group and the controls. RESULTS: All of the BMPRs proteins were expressed in the human benign prostate tissues, with various concentrations. The semiquantitative PCR analysis showed that the mRNA level of the hBMPRs tended to decrease following 5alpha-reductase inhibition, and the magnitude of this decrease was the greatest for the hBMPR-IB. CONCLUSIONS: In the human prostate, only the expression of hBMPR-IB was significantly reduced by the suppression of DHT. Further studies on the possible role of the hBMP-hBMPR-IB complex, in the abnormal proliferation of the prostate under physiological conditions, are warranted.
