Effect of caspase-3 on the primary cultured mice cortical neurons with herpes simplex virus type-1 infection
- VernacularTitle:单纯疱疹病毒1型感染对原代培养鼠皮质神经元凋亡caspase-3途径的影响
- Author:
Jiawei WANG
;
Dexin WANG
;
Zijing FENG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Caspases;
Herpes simplex;
Neurons;
Apoptosis in autotranspla ntated splenic tissue
- From:
Chinese Journal of Neurology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of caspase-3 on the primary cultured mice cortical neuron w ith herpes simplex virus type 1 (HSV-1) infection. Methods A spectrophotometer was used to de tect the absorbance units at 405 nm of the caspase-3 substrate,the chrom o phore p-nitroanilide(pNA ).Comparing with the normal group, virus group, s orbital group,and sorbital group with virus in the absorbance units of chromophore substrate.The changes in inner mitochondrial membrane p otential of the different group cells were analyzed with flow cytometry. Results The released chromphore was measured by determining the absorbance at 405 nm. N group is 0.394 2, V group is 0.240 8, NS group is 0.539 2, VS gr oup is 0.465 0,the control C1 group is 0.290 0, C2 group is 0.235 0 and all groups were analyzed by statistic (P
