The study of urethral epithelium culture in vitro
- VernacularTitle:尿道粘膜上皮细胞的分离培养和鉴定
- Author:
Senkai LI
;
Hongfeng ZHAI
;
Yangqun LI
;
Al ET
- Publication Type:Journal Article
- Keywords:
Urethra;
Epithelium;
Cell,culture
- From:
Chinese Journal of Urology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study urethral epithelium cu lt ure in vitro as a basic role in the further study on tissue engineering urethra. Methods A bit of urethral mucosa was taken from a young male New Zealand hare and was digested by enzyme into single cell liquid.The ce ll was then cultured in a still condition.The medium was renewed regularly and t he cells were subcultured and studied. Results All the c utured cells were urethral diploid cells without fibroblasts.The cells could be subcultured 11～13 generations with a surviving period of 50～60 days. Conclusions The urethral epithelium the cultured cells of young N ew Zealand hare can be cultured in vitro and is able to proliferate within a cer tain period.This provides a strong foundation for the further study on tissue en gineering urethra.
