Identification of the antigenic determinants of human augmenter of liver regeneration (hALR) recognized by its monoclonal antibodies
- VernacularTitle:抗人肝再生增强因子抗体识别部位的确定
- Author:
Bo ZHANG
;
Huipeng CHEN
;
Qingming WANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Human augmenter of liver regeneration Monoclonal antibodies Antigenic deteminant Competitive enzyme linked immunosorbent assay
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Identification of the antigenic determinants of hALR.Methods:Theoretic antigenic determinants of hALR was predicted by using Hopp&Wodds method and and Goldkey software package.The four polypeptides according to the amino acid sequences of the predictive linear epitopes of hALR were synthesized and were used to analyze the antigenic determinants of hALR recognized by antibodies.Results:The polypeptides corresponding to residues 6～5,68～80 and 105～112 of hALR were its antigenic determinants.Conclusion:Prediction of the protein antigenic determinants by computer program and detection of them by competitive ELISA with synthesized polypeptides is a useful method of identification of the antigenic determinants.
