Cloning aged-related gene of mouse thymus
- VernacularTitle:小鼠增龄相关性胸腺基因的克隆
- Author:
Yonghai LI
;
Hua SU
;
Wei HE
;
Al ET
- Publication Type:Journal Article
- Keywords:
DDRT PCR Mouse thymus Gene expression difference
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Differential expression analysis and cloning aged related genes of mouse thymus Methods:Different expressions of thymus mRNAs from 1 and 10 month old mouse were analyzed by DDRT PCR and different expression sequence tags (ESTs) were obtained One EST that represented high expressed level in one month mouse thymus was probed to screen mouse thymus cDNA library One 827 bp cDNA fragment was obtained and was extended to 1 406 bp by PCR Results:Homology analysis showed that mt22 1406 contained one 438AA coding region and showed high similarity with human elongation factor1?(EF1?) The Genbank accession number is BE241062 Conclusion:Cloning one gene related with mouse thymus aging
