The effect of secreted and transmembrane stem cell factor on biological activities of mast cells
- VernacularTitle:跨膜型SCF与分泌型SCF对肥大细胞生物学功能影响的研究
- Author:
Hao ZHENG
;
Zhuoya LI
;
Feili GONG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Stem cell factor Mast cell Proliferation Histamine Tumor necrosis factor ? mRNA
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of secreted stem cell factor(S SCF) and transmembrane SCF(TM SCF) on the biological functions of mast cells,including proliferation,release of histamine and secretion of cytokine to explore the biological characteristics of the two forms of SCF.Methods:MTT,fluorescence technique,in situ hybridization were used to detect the several biological functions of mast cells,such as proliferation,release of histamine and the transcription of TNF ? mRNA.Results:It was shown that S SCF could significantly promote the proliferation of mast cells,release of histamine and TNF ? mRNA transcription,while TM SCF had only a weaker positive effect on histamine relase and no any effect on proliferation and TNF ? mRNA transcription.Conclusion:The biological actions of TM SCF on mast cells are different from that of S SCF.
