Construction of recombinant fowlpox virus with HIV-1Gag-gp120 fusion protein
- VernacularTitle:含中国流行株HIV-1Gag-gp120融合蛋白的重组鸡痘病毒的构建
- Author:
Ningyi JIN
;
Kun LUO
;
Houhua FANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
HIV 1 Gag gp120 Recombinant fowlpox virus
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To express Gag gp120 fusion protein in the recombinant fowlpox virus.Methods:The Gag gp120 gene of HIV 1 was inserted downstream of the combined promoter in pUTAL,an expressing plasmid was constructed.By transfecting the plasmid into CEF cells pre infected with FPV 282E 4 strain,a recombinant fowlpox virus vUTALG was obtained by selecting in the presence of BUdR and subsequent blue plaque screening.Results:Western blot showed that the recombinant virus expressed the Gag gp120 fusion protein in the infected CEF cells.The virus like particles formed by Gag protein were observed under electron microscope.Mice were immunized with the recombinant virus.The results showed the recombinant virus could induce HIV 1 specific antibody response.Conclusion:The recombinant fowlpox virus could express the Gag gp120 fusion protein.
