Purification and functional assay of recombinant human interleukin 18
- VernacularTitle:重组人白细胞介素18的纯化及其生物学活性研究
- Author:
Ruijun ZHANG
;
Zhigang TIAN
;
Haiming WEI
;
Al ET
- Publication Type:Journal Article
- Keywords:
Interleukin 18 Purification Activity assay
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To purify recombinant human interleukin 18(rhIL 18) and perform activity assay of renatured rhIL 18 Methods:The rhIL 18 was purified by molecular sieve chromatography MTT assay and RT PCR method were used in activity assay of rhIL 18 Results:The purity of rhIL 18 was up to 96% The renatured rhIL 18 could enhance the cytotoxicity of NK cells and induce IFN ? gene expression in PBMC Conclusion:The rhIL 18 prepared exerts functional activity and can be applied for further studies
