The study of the effective mechanism of infected host serum on the infectivity of gametocytes of Plasmodium yoelii (P.yoelii)
- VernacularTitle:约氏疟原虫感染血清对疟原虫配子体感染力作用机制的研究
- Author:
Yaming CAO
;
Yingjie LIU
;
Chao LV
;
- Publication Type:Journal Article
- Keywords:
Plasmodium yoelii(P.yoelii) Crisis serum Nitric oxide Infectivity of gametocyte
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the mechanism of crisis serum' mediated gametocyte infectivity to the mosquito vector Methods:Observing the effects of mouse serum , which was obtained 5 days after P yoelii infection (D5 serum) on gametocyte infectivity by IFA and mosquito live feeds, and the production of IFN ??TNF ??IL 4 and NO - 2 in the hosts in vivo and in vitro by ELISA and Griess reaction And to investigate the ability of malaria parasitized red blood cell extract (PRBC extract) to induce NO Results:The development of the gametocytes from mice 5 days postinfection into ookinetes were completely inhibited D5 serum was not immediate to inhibit gametocyte development, which was injected intravenously into the mice 3 days after P yoelii infection But 4 h later after injection D5 serum stimulated the increasing IFN ? and NO production and inhibited gametocyte infectivity Moreover, PRBC extract showed the ability to induce NO Conclusion:Infected host serum blocks transmission of P yoelii via a nitric oxide dependent mechanism
