The effects of FL、rIL-6、rIL-6R in vitro expansion and differentiation of human cord blood CD34~+ cells
- VernacularTitle:FL、rIL-6、rIL-6R对脐血CD34~+细胞的体外分化增殖作用
- Author:
Yang WANG
;
Meirong ZHAO
;
Yiduo XU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Cord blood CD34 + Hematopoietic stem cell
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the effects of FL,rIL 6,rIL 6R in vitro expansion and differentiation of human cord blood CD34 + cells.Methods:Human cord blood CD34 + cells were enriched by magnetic cell isolation system CD34 + cells were incubated in the presence of FL,rIL 6,rIL 6R and their various companision.The total number of cells and colonies were counted at various time intervals.Results:The expanded cells by FL,rIL 6,rIL 6R showed colonies were CFU GM mainly but rIL 6+rIL 6R and FL+rIL 6+rIL 6R,showed some BFU E and CFU Mix but no CFU MK.Erythrocyte cells were conformed and total cells were increased 6.4 folds by FL+rIL 6+rIL 6R.Conclusion:Human cord blood CD34 + cells could expanded and differentiated by FL+rIL 6+rIL 6R.
