Expression and purification of Helicobacter pylori urease A subunit and catalase
- VernacularTitle:幽门螺杆菌尿素酶A、过氧化氢酶的表达及纯化
- Author:
Wenjun LIAO
;
Minhu CHEN
;
Senlin ZHU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori Vaccine KatA Urease
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Investigated the effect of recombinant urease A subunit and catalase vaccines on protection against Helicobacter pylori (HP) infection Methods:Recombinant plasmids expressing UreA or KatA were constructed Expression was induced with IPTG,analyzed by SDS PAGE and Western blot UreA and KatA were purified with Bulk GST purification module Results:The recombinant plasmids could express GST UreA and GST KatA fusion proteins,which accounted for 35% and 19% of total bacteria proteins respectively,and could specially react with antibody against GST The purity of the purified UreA and KatA was over 95% Conclusion:The work laid a foundation for further studies of HP vaccine The recombinant vaccines may play an important role in preventing and treating HP infection and related diseases
