Study on HL-60 cell apoptosis induced by CD3 McAb-activated killer cells in serum-free culture system
- VernacularTitle:无血清培养体系中CD3AK细胞诱导HL-60细胞凋亡的研究
- Author:
Guangjin LI
;
Gongxing CHEN
;
Weiyao YAN
;
Al ET
- Publication Type:Journal Article
- Keywords:
Serum free culture Anti CD3 monoclonal antibody Apoptosis Granzyme
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether CD3McAb activated killer(CD3AK)cells could induce apoptosis in HL 60 cells in serum free culture system.Methods:HL 60 cells were coincubated with CD3AK cells expanded in serum free medium,then apoptosis was assessed by means of morphological studies,DNA agarose gel electrophoresis and flow cytomether(FCM) analysis.Results:After 7 h of coincubation with CD3AK cells in serum free medium,HL 60 cells showed morphological features of apoptosis and typical DNA ladder on gel electrophoresis.FCM analysis showed that 24.59% cells were found to undergo apoptosis.Conclusion:CD3AK cells can induce apoptosis in HL 60 cells under serum free culture conditions.
