The Change of Histologic Features and Eicosanoids Level in Rat Testicular Ischemia-Reperfusion Injury.
- Author:
Suk Jai LEE
1
;
Gil Ho LEE
;
Hyung Jee KIM
;
Hyung Gun KIM
;
Eo Jin KIM
Author Information
1. Department of Urology, Dankook University College of Medicine, Cheonan, Korea. gilho@anseo.dankook.ac.kr
- Publication Type:Original Article
- Keywords:
Testicular ischemia-reperfusion injury;
Histologic features;
Prostaglandins;
HPLC
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Dinoprostone;
Eicosanoids*;
Humans;
Ischemia;
Male;
Prostaglandins;
Rats*;
Rats, Sprague-Dawley;
Reperfusion;
Reperfusion Injury*;
Spermatic Cord;
Thromboxane B2
- From:Korean Journal of Urology
2002;43(12):1086-1092
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: It has been suggested that prostaglandins (PGs) play an important role in ischemia-reperfusion (IR) organ injury. However, the significance of PGs is not well documented for a testicular IR injury. The purpose of this study was to assess the potential role of PGs in rat testicular ischemia and IR injury MATERIALS AND METHODS: Sprague-Dawley male rats were divided into 7 groups, each containing 7 rats. The right spermatic cord was clamped with vascular clamps for 30 minute (group B, C), 90 minute (D, E) and 240 minute (F, G). Groups B, D, and F were ischemia groups and the group C, E, and G were reperfusion groups. Reperfusion was carried out after ischemia lasting 30, 90 and 240 min. The levels of thromboxane B2 (TxB2), 6-keto-PGF1alpha and prostaglandins E2 (PGE2) were measured using High-Performance Liquid Chromatography (HPLC). RESULTS: The levels of TxB2 were significantly elevated in groups D, E, F, and G than in the controls (p<0.05, each group). The TxB2 levels in the ischemia group F were significantly decreased compared to that of group D (p<0.05). The histological features shown in groups D, and F were typical ischemic changes, but atypical in groups B, and C. The most severe damage was noted in group G. These features were well matched with the changes of TxB2 in testicular ischemia and IR injury. The levels of 6-keto-PGF1alpha were significantly elevated in all the ischemia and IR groups compared to that of the controls (p<0.05). CONCLUSIONS: These results suggested that TxB2 might have an active role in testicular IR injury, although PGE2 and 6-keto-PGF1alpha seemed not to have an active role.
