Purification and bioactivity detection of recombinant human IL-15
- VernacularTitle:重组人白细胞介素15的纯化及生物活性鉴定
- Author:
Anyuan SUN
;
Rui SUN
;
Zhigang TIAN
;
Al ET
- Publication Type:Journal Article
- Keywords:
rhIL 15 Purification Bioactivity
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Studies on purification and bioactivity detection of recombinant human IL 15 protein.Methods:The rhIL 15 were purified from rhIL 15 producing DH5? bacteria the inclusion bodies extracted first and followed by gel filtration,ion exchange chromatography and reverse phase high performance liquid chromatography(RP HPLC).The bioactivities were detected by NK cytotoxicity.Results:Recombinant human IL 15(rhIL 15)was obtained with a purity of 99 percent.The N terminal amino acid sequence of the rhIL 15 was as same as the natural IL 15.The specific activity of the rhIL 15 is 1?10 7 U/mg,and the rhIL 15 significantly enhances NK cytotoxicity.Conclusion:Recombinant human IL 15 of high purity and bioactivity could be obtained by three steps of purification.The high quanity and quality of the rhIL 15 is important to both the laboratory research and the clinical trial.
