Induction of CD45 expression in a myeloma cell line by IL-6
- VernacularTitle:IL-6诱导人骨髓瘤细胞表达CD45
- Author:
Lun SONG
;
Yan LI
;
Yingxun SUN
;
Al ET
- Publication Type:Journal Article
- Keywords:
Myeloma IL 6 Signal transduction CD45
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between “activation of IL 6 signal transduction pathways” and “induction of CD45 expression” in a myeloma cell line—Sko 007 by IL 6.Methods:Electrophoretic mobility shift assay(EMSA) was used to detect the activation of the transcription facotrs STAT3 and NF IL 6 by IL 6,which can represent the activation state of the two IL 6 signal transduction pathways respectively;Then the induction of CD45 mRNA and protein expression by IL 6 were detected by RT PCR and FACS.The effect of antisense expression plasmid of STAT3 pAT STAT3 on STAT3 activation and CD45 expression was shown by EMSA and RT PCR,respectively.Results:①Both two IL 6 signal transduction pathways JAK/STAT and Ras/NF IL 6 can be activated by IL 6 in Sko 007 cells.②The expression level of CD45 mRNA and protein is Sko 007 cells can be up regulated by IL 6.③The activation of JAK/STAT signal transduction pathway and induction of CD45 mRNA expression by IL 6 can be inhibited in pAT STAT3 transfected Sko 007 cells.Conclusion:IL 6 induced CD45 expression in Sko 007 cells in mediated by the activation of JAK/STAT signal transduction pathway.
